Operations & Maintenance Courses

Operations and maintenance courses are designed for maintenance staff supporting the activities of BSL-2 and BSL-3 laboratories and have responsibilities for the operational systems of these laboratories. 

Course: NBB-100 - Introduction to Biosafety and Biocontainment for Operations and Maintenance Professionals (0.4 CEU)

Prerequisite(s): None

Summary: Introduction to biocontainment laboratory philosophy and design intent. Explanation of the distinguishing characteristics between BSL-1, BSL-2, BSL-3 (high containment) and BSL-4 (maximum containment) laboratories.

Learning Outcomes: The participant will be able to:

Develop working knowledge of contents of the BMBL 5th Edition
Describe what is meant by biocontainment philosophy and design intent
Discuss the characteristics of BSL-1, BS-2, BSL-3 & BSL-4 laboratories
Explain the laboratory design process

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Course: NBB-110 - Fundamentals of Laboratory Mechanics (0.4 CEU)

Prerequisite(s): NBB-100

Summary: Overview of basic requirements for biosafety laboratories including architectural and mechanical components, operational characteristics, and routine maintenance issues. Includes basic design parameters outlined in 5th edition of the BMBL

Learning Outcomes: The participant will be able to:

Define basic containment laboratory ventiliation terms and ventilation equations
Explain thermodynamic elements in a biocontainment laboratory
Explain the psychometric properties of air
Explain the principles of ventiliation control and airflow
Discuss lab pressure relationship requirements

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Course: NBB-120 - Laboratory Systems, Utilities and Maintenance I (0.4 CEU)

Prerequisite(s): NBB-110

Summary: Review of BMBL guidelines for design and operation of biosafety laboratories. In-depth discussion of laboratory utility systems and system requirements including: electrical, vacuum, CO2, nitrogen, compressed air, plumbing and waste processing, disinfection, and decontamination. Also covered are topics concerning back-up and redundant system requirements, chemical handling and storage, laboratory security procedures, and routine, planned and preventive maintenance issues.

Learning Outcomes: The participant will be able to:

Explain how HEPA filters work
Discuss biocontainment requirements for electrical, plumbing, security, and waste systems
Describe lightning protection and emergency power systems
Analyze laboratory layouts for personnel and product flow as well as airflow and pressure relationships

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Course: NBB-130 - Overview of Microbiology and Biosafety for Operations and Maintenance Professionals (0.4 CEU)

Prerequisite(s): None

Summary: Introduction to basic principles of microbiology and biosafety.

Learning Outcomes: The participant will be able to:

Describe the chain of infection
Discuss what is meant if someone says a 'bug' is pathogenic or non-pathogenic; virulent or non-virulent
Describe the basic differences between bacteria, fungi, parasites, viruses and prions
Define "Biosafety Level"

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Course: NBB-140 - Laboratory Sterilization and Disinfection for Operations and Maintenance Professionals (0.4 CEU)

Prerequisite(s): None

Summary: Decontamination and sterilization requirements and practices in the biocontainment laboratory environment. Includes discussions of various disinfection systems, chemicals utilized in the disinfection process, chemical handling and storage requirements, and an overview of waste processing systems.

Learning Outcomes: The participant will be able to:

Become familiar with the vocabulary of disinfection and sterilization
Define disinfection and sterilization
Identify two disinfectants that are likely to be used in your workplace
Identify two methods of sterilization that may be encountered in your workplace
Describe the chain of infection
Identify the basic differences among bacteria, fungi, parasites, viruses and prions
Identify the differences between pathogenic and non-pathogenic and virulent and non-virulent organisms

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Course: NBB-200 - Laboratory Systems, Utilities and Maintenance II (0.4 CEU)

Prerequisite(s): NBB-120

Summary: In-depth discussion of architectural components of laboratory design and construction including material requirements and architectural fit and finish details; reading and understanding architectural and mechanical drawings; issues and concerns related to new construction versus renovated spaces; security and safety topics including fire protection and emergency response and preparedness; and maintenance record keeping requirements. Review of BMBL guidelines for design and operation of biosafety laboratories.

Learning Outcomes: The participant will be able to:

Read and interpret architectural drawings
Explain maintenance record keeping requirements

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Course: NBB-210 - Troubleshooting Laboratory HVAC, Filtration, and Utility Systems (0.4 CEU)

Prerequisite(s): NBB-200

Summary: Troubleshooting laboratory system components and operation including architectural (seals, bioseals, finishes, doors, locking mechanisms), mechanical (air handling units, refrigeration systems, fans, filters, controls, BAS systems), electrical (power, lighting, low voltage control systems), and plumbing and waste systems (drains, small pipe utilities, waste processing systems).

Learning Outcomes: The participant will be able to:

Troubleshoot architectural and engineering components of biocontainment labs to ensure continuous system performance
Develop preventive maintenance programs for biocontainment laboratories

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Operations & Maintenance - Elective Courses

Course: NBB-220 - Biocontainment Laboratory Architecture & Design (0.4 CEU)

Prerequisite(s): None

Summary: In-depth investigation into the elements that culminate in the successful design of a biosafety laboratory. Topics include space planning, programming, functional design relationships, design constraints, and adherence to BMBL guidelines.

Learning Outcomes: The participant will be able to:

Become familiar with resources for biocontainment laboratory design
Identify the design requirements for a biocontainment laboratory
Identify design criteria differences between BSL-3 and BSL-4
Describe design considerations by zone
Identify design and construction issues

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Course: NBB-300 - Emerging Issues in Biosafety and Biocontainment for O&M Professionals (0.4 CEU)

Prerequisite(s): NBB-200

Summary: Panel discussion of the emerging issues in biosafety and biocontainment.

Learning Outcomes: The participant will be able to:

Analyze emerging issues in biosafety and biocontainment
Describe how these issues will affect operations and maintenance
Explain how the OM staff person's role may be changed or affected

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Course: NBB-310 - Laboratory Construction Issues(0.4 CEU)

Prerequisite(s): NBB-110

Summary: Rigorous introduction to the construction issues surrounding biosafety containment laboratories. Issues and topics include construction methods, establishing air-tight seals, boundary/barrier penetrations, acceptable products and materials, etc.

Learning Outcomes: The participant will be able to:

Under Development

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Course: NBB-320 - Laboratory Commissioning and Certification(0.4 CEU)

Prerequisite(s): NBB-110

Summary: Detailed study of the process of commissioning and certifying a biosafety laboratory.

Learning Outcomes: The participant will be able to:

Explain the elements of commissioning and NIH certification
Describe the roles and goals of commissioning
Describe roles and goals of certification
Describe how to perform FPT - Functional Performance Testing
Discuss NIH Certification Acceptance Criteria

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Course: NBB-330 - Laboratory Systems: Continuity of Operations Planning(0.4 CEU)

Prerequisite(s): NBB-110

Summary: Introduction to business continuance planning for biosafety laboratories. Discussion will include the benefits of developing a continuance of operations plan (COOP) and the structural elements that comprise such a plan. COOP scenarios will include environmental disasters, organized and/or deliberate disruption, loss of utilities and services, equipment and/or system failures, serious information security incidents, and other emergency situations.

Learning Outcomes: The participant will be able to:

Describe several risk reduction techniques
Describe how to perform risk assessment
Explain how to develop failure scenarios
Explain requirements of COOP
Describe the benefits of developing a COOP
Demonstrate understanding of structural elements of COOP
Analyze various COOP scenarios

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